RAA Basic Nucleic Acid Amplification Kit (RUO)
Rapid amplification of DNA at 37 °C; product confirmed by agarose gel electrophoresis. Runs on a heat block, water bath or incubator.
DNAGel readout48 tests50 μL
RAA · Recombinase-Aided Amplification
Rapid nucleic acid testing, without the thermal cycler.
RAA runs isothermally at 37–42 °C and returns a fluorescence result in 5–15 minutes. Lyophilised reagents, a portable analyzer and a two-step workflow take molecular testing to the field. All reagents are for research use only.
5–15 minTime to fluorescence result
37–42 °CIsothermal, no cycling
2 stepsExtract, then amplify & read
5–15 minFluorescence readout, typically within 15 minutes of starting the reaction
30–42 °CIsothermal reaction range (optimum 37–39 °C); heat block, water bath or analyzer
1 %Limit of detection for meat-species adulteration (w/w)
103 cfu/mLLimit of detection for foodborne pathogen kits
RAA Technology
Isothermal amplification, driven by a recombinase.
RAA (Recombinase-Aided Amplification) is the isothermal amplification platform behind every ZC Bioscience reagent. All kits are developed and manufactured in-house.
How it works
Recombinase-Aided Amplification is a rapid isothermal nucleic acid amplification technology. A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand, and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.
With a labelled fluorescent probe the reaction can be read in real time; a fluorescence result is typically available within 5–15 minutes. All reaction components are pre-mixed and lyophilised in the tube — simply add Buffer A, Buffer B and the extracted sample DNA. RAA can in principle be multiplexed: two or more primer pairs in one reaction amplify several targets simultaneously, saving reagents and cost.
RAA is applied across food safety (pathogen detection, meat-species authentication, GMO screening), aquaculture pathogen detection, animal disease detection and other fields.
Reaction sequence
One tube, one temperature
01
Recombinase binds primerForms a recombinase–primer complex at ambient temperature.
02
Complex scans the templateInvades the duplex and pairs at the fully complementary site.
03
SSB holds the strand openSingle-stranded DNA-binding protein stabilises the opened template.
04
Polymerase extendsStrand-displacing DNA polymerase synthesises the new strand; product grows exponentially.
05
Probe reports in real timeExonuclease-cleaved fluorescent probe gives a curve on the Genchek analyzer, or read by gel or lateral-flow strip.
37–39 °C optimum
Primers 30–35 bp
Probes 46–52 bp
>107-fold amplification
Six advantages of the platform
Simple operation
Molecular-level testing that does not require specialist staff. Two-step workflow: extract, then amplify and read.
Fast
Results in 5–15 minutes; truly rapid testing that fits high-volume, short-shelf-life sample streams.
Accurate
High sensitivity and specificity; the recombinase–primer complex recognises the target sequence by base pairing.
Portable
Compact instruments suited to harsh field conditions and on-site inspection; the analyzer weighs 3.8 kg.
Isothermal
Runs at 30–42 °C; no thermal cycler or other expensive equipment required — a heat block or water bath is enough.
Lyophilised
Reagents supplied as freeze-dried pellets, ready to use, easy to ship, and free from freeze–thaw loss.
| Platform comparison | PCR | LAMP | RAA |
|---|---|---|---|
| Enzymes | Taq DNA polymerase | Strand-displacing DNA polymerase | Recombinase, single-stranded DNA-binding protein, strand-displacing DNA polymerase |
| Reaction temperature | High temperature, thermal cycling | 60–65 °C | 37–39 °C |
| Reaction time | 1.5 h | 40 min | 15 min |
| Primer pairs | 1 | 2 | 1 |
| Reagent format | Liquid | Liquid | Lyophilised pellet |
| Readout | Gel; real-time fluorescence | Visual turbidity | Gel; real-time fluorescence; lateral-flow strip |
| Equipment | Thermal cycler | Heat block at 60–65 °C | Heat block, water bath or portable analyzer |
Typical values from ZC Bioscience method comparison. Actual run times depend on target, sample matrix and readout.
Products
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Five product lines, one isothermal platform.
29 catalogue items: core amplification kits for assay developers, ready-to-run food-safety kits, extraction reagents and the Genchek instruments that read them. All reagents are for research use only (RUO).
Category 01 · 6 products
RAA Core Amplification Kits
Enzyme reagent kits for building your own RAA assay. Pre-mixed, lyophilised, 48 tests per kit in a 50 μL system. Choose DNA or RNA (RT) chemistry and gel, real-time fluorescence or lateral-flow readout.
RAA Fluorescent Nucleic Acid Amplification Kit (RUO)
Rapid DNA amplification with real-time monitoring on the Genchek analyzer or standard real-time PCR instruments; 5–20 min.
DNAReal-time48 tests50 μL
RT-RAA Basic Nucleic Acid Amplification Kit (RUO)
Rapid amplification of RNA — reverse transcription and RAA in one tube at 37 °C, with gel readout.
RNAGel readout48 tests50 μL
RT-RAA Fluorescent Nucleic Acid Amplification Kit (RUO)
Rapid amplification of RNA with real-time fluorescence readout on the Genchek analyzer; 5–20 min.
RNAReal-time48 tests50 μL
RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)
Rapid DNA amplification at 39 °C; the nfo-cleaved probe and biotin-labelled primer yield a dual-labelled amplicon read on a universal lateral-flow strip.
DNAStrip readout48 tests50 μL
RT-RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)
Rapid RNA amplification at 42 °C with lateral-flow strip readout — no instrument required beyond a heat block.
RNAStrip readout48 tests50 μL
Category 02 · 1 instrument
Genchek Isothermal Fluorescence Analyzer
An integrated, portable rapid-test analyzer for on-site and mobile testing. Built-in reagent mixing, fully matched to RAA reagents; dual fluorescence channels (FAM & ROX); automatic data analysis and report generation with multiple export options.
Genchek Isothermal Fluorescence Analyzer
Portable 8-well analyzer for real-time RAA fluorescence detection. Laptop-sized, 3.8 kg, with built-in mixing and on-board reporting.
8 wellsFAM / ROX3.8 kgAC 100–240 V
| Throughput | 8 wells |
| Fluorescence channels | FAM / ROX |
| Temperature range | Ambient to 65 °C |
| Temperature accuracy | ±0.5 °C |
| Net weight | 3.8 kg |
| Power | 120 W |
| Dimensions (L×W×H) | 255 × 240 × 123 mm |
| Power supply | AC 100–240 V, 50/60 Hz |
| Operating system | OPENRTOS |
| Operating environment | 5–35 °C, 20–80 % RH |
| Storage environment | -20 to 55 °C, <90 % RH |
| Compatible reagents | All ZC Bioscience fluorescent RAA kits |
Category 03 · 4 products
Rapid Nucleic Acid Extraction Kits
Purpose-built front ends for RAA: heat-lysis in 5 minutes, spin columns in 10–15 minutes, or a magnetic-bead plate for automated extractors. No hazardous solvents, low cross-contamination risk.
Rapid DNA Extraction Reagent, Heat-Lysis (RUO)
5-minute genomic DNA from animal tissue, blood, meat products, plant tissue, seeds and bacteria. Surfactant/chemical lysis — no organic extraction, no ethanol precipitation.
Heat lysis5 min50 preps
Universal Rapid DNA Extraction Kit, Spin Column (RUO)
Lyse–bind–wash–elute in 15 minutes. Animal tissue, cells, bacteria, saliva, blood, swabs, faeces, bone and feed; high yield and stable quality.
Spin column15 min50 preps
Universal Rapid RNA Extraction Kit, Spin Column (RUO)
Four steps, 10 minutes, high-quality RNA from animal tissue, cells, saliva, blood, swabs and faeces — ready for RT-RAA.
Spin column10 min50 preps
Magnetic-Bead DNA Extraction Pre-filled Plate (RUO)
For automated magnetic-bead extractors. Nano-magnetic-bead purification with high yield and consistency; cuts hands-on time for food-safety, environmental microbiology, livestock and molecular biology research.
Magnetic beads25 min96 preps
Category 04 · 17 products
Food Safety Molecular Detection Series
Ready-to-run RAA fluorescent kits, 24 tests per kit. Result read directly from the amplification curve and time — no electrophoresis. Compatible with Genchek-I/II and standard real-time PCR instruments (ABI, MJ, Roche, Bioer series).
Animal-Derived Ingredient Molecular Detection Kits (RAA, Fluorescent) (RUO)
Species-specific gene detection in animal tissue, blood, foods, meat products (including processed meat) and feed. Each kit detects different breeds of its target species.
Chicken · A101ZCDuck · A102ZCPork · A103ZCBovine · A104ZCSheep & goat · A105ZCRat · A106ZCHorse · A107ZCMink · A108ZCFox · A109ZCDonkey · A110ZC
24 tests/kitLOD 1 % (w/w)30–42 °C
Foodborne Pathogen Molecular Detection Kits (RAA, Fluorescent) (RUO)
Qualitative detection of pathogens in foods, beverages, water, cosmetics, disposable hygiene products and seafood. Method references: GB 4789 series and SN/T series.
Staphylococcus aureus · B201ZCSalmonella · B202ZCPseudomonas aeruginosa · B203ZCListeria monocytogenes · B204ZCHaemolytic Streptococcus · B205ZCVibrio parahaemolyticus · B206ZCShigella · B207ZC
24 tests/kitLOD 103 cfu/mL5–15 min
GMO Molecular Detection Kits (RAA, Fluorescent) (RUO)
Screening kits for six common transgenic elements, plus event-specific kits for soybean, maize and canola lines. 24 tests per kit; catalogue numbers and availability on request.
CaMV 35S promoterFMV 35S promoternos terminatorBt genebar genehpt geneSoybean GTS 40-3-2Soybean MON89788Maize MON810Maize Bt11Canola T45Canola Oxy-235
Category 05 · 1 instrument
Genchek Fully Automated Isothermal Nucleic Acid Detection System
Fully automated, sample-in/result-out in 30 minutes. Performs pipetting, lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting in one instrument.
Product photography available on request
Genchek Fully Automated Isothermal Nucleic Acid Detection System
Benchtop automation for RAA: quantitative pipetting, sample lysis, purification, amplification and dual-channel fluorescence detection with automatic reporting.
Sample-in / result-out~30 minFAM / ROX16 kg
| Sample throughput | 1 per run |
| Run time | Approx. 30 min |
| Channels | FAM / ROX |
| Interface | 7-inch colour touchscreen |
| Weight | 16 kg |
| Rated power | 221 W · AC 220 V, 50 Hz |
| Dimensions (L×W×H) | 260 × 325 × 420 mm |
| <15 μL | Accuracy >98 %, CV <5 % |
| 15–50 μL | Accuracy >98.5 %, CV <3 % |
| >50 μL | Accuracy >99 %, CV <1.2 % |
| Operating environment | 20–30 °C, 20–80 % RH |
| UV lamp / air purification | Yes / Yes |
Solutions · Food Safety
Three application areas, one workflow.
Isothermal RAA reagents specifically recognise and amplify the target gene fragment; the Genchek analyzer monitors amplification in real time and the run completes in 15 minutes.
10 speciesLOD 1 % adulteration5–15 min
Meat Species Authentication
As price gaps between meats widen, species substitution and adulteration are increasingly common. Short shelf lives and high sample volumes call for a fast, accurate and practical method. RAA-based species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction between species.
Targets
ChickenPorkDuckBeefSheep & goatRatHorseMinkFoxDonkey
Users — food and drug regulators, customs and border inspection, livestock authorities, agricultural research institutes, law enforcement; food, dietary-supplement and feed manufacturers, third-party testing laboratories.
9 targetsLOD 103 cfu/mLMulti-channel
Foodborne Pathogen Detection
In China, pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases each year. ZC Bioscience's RAA pathogen kits combine multi-channel detection, short run times and high sensitivity — raising testing throughput, moving food-safety warnings earlier and cutting labour cost. Qualitative detection in food, beverages, cosmetics and seafood.
Targets
S. aureusSalmonellaP. aeruginosaL. monocytogenesHaemolytic StreptococcusV. parahaemolyticusShigellaE. coli O157:H7V. cholerae
Users — food and drug regulators, customs and border inspection, public-health laboratories; food and cosmetics manufacturers, third-party testing laboratories. Method references: GB 4789 series, SN/T series.
6 elementsEvent-specific kitsReal-time
GMO Screening
GM crops carry genes introduced from other organisms to alter traits, nutrition or quality. Alongside their economic value they raise questions of food safety and environmental impact, and regulators worldwide require labelling and control to protect consumers' right to know. ZC Bioscience's RAA-based GMO kits specifically detect exogenous elements and events in crops, identifying GM material at the molecular level.
Targets
BtbarhptCaMV 35S promoterFMV 35S promoternos terminator
Users — food and drug regulators, customs and border inspection, livestock authorities, agricultural research institutes; food, dietary-supplement and feed manufacturers, third-party testing laboratories.
Example workflow
From 50 mg of sample to a result in under 30 minutes.
Animal-species assay on the Genchek analyzer. RAA is simple to run and needs no specialist; two pipetting steps separate the sample from the answer.
10 min
STEP 01
ExtractWeigh 50 mg of sample into the extraction solution and incubate at 70 °C for 10 minutes.1 min
STEP 02
RehydrateAdd 45.5 μL Buffer A, 2.5 μL Buffer B and 2 μL sample nucleic acid to the lyophilised reagent tube.5–15 min
STEP 03
Amplify & readPlace the tube in the Genchek analyzer. The amplification curve reports the result in 5–15 minutes.
Services
Support that follows the reagent into the field.
Our R&D team answers every product question with patient explanation and demonstration — consultation, technical documentation, training and after-sales support, plus custom RAA assay development.
Technical support
Expert technical support from the people who develop the chemistry: consultation on target and sample type, technical documentation, method demonstration and operator training.
After-sales service
Good after-sales service earns customer trust. We provide quality after-sales service with long-term technical documentation and support. If a problem arises, our engineers respond promptly and resolve issues online or by phone; where an on-site visit is needed we agree the arrival time with you.
Custom assay development
RAA is a versatile, portable rapid-testing platform that can replace LAMP or PCR in many workflows. Our products are continuously improved, and we also develop custom RAA assays to meet specific customer needs — contact us to discuss your target.
About ZC Bioscience
An R&D-driven biotechnology company in Hangzhou.
Hangzhou ZC Bio-Sci & Tech Co., Ltd. ("ZC Bioscience") is an R&D-driven biotechnology company based in Hangzhou, China. We develop and apply Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supply RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing. All reagents are for research use only.
Since September 2016 the company has accelerated product development for RAA-based rapid food-safety testing, launching in turn the animal-species (meat authentication) molecular detection series, the foodborne pathogen molecular detection series and the GMO ingredient molecular detection series. Other application areas include environmental monitoring, aquaculture pathogen detection and animal disease detection.
ZC Bioscience has signed strategic cooperation agreements with government institutions and research organisations across several fields to co-develop RAA-based detection kits. We remain committed to advancing RAA technology and delivering rapid-testing products and services that are fast, accurate and easy to use.
500 m²Laboratory space, plus 400 m² of offices
RMB 12M+Installed laboratory equipment
90 %+Of the R&D team hold master's degrees or higher
5050Selected for the Hangzhou Binjiang District "5050 Talent Program"; a key enterprise cultivated by the district
News
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Company news.
Explainer | Recombinase-Aided Rapid Nucleic Acid Amplification
RAA is an isothermal in-vitro amplification method in which recombinase, single-stranded DNA-binding protein and DNA polymerase replace the thermal-cycling denaturation of conventional PCR, allowing rapid amplification at a constant 37 °C.
Read more →
Highlights | RAA Technology Debuts at the Food Microbiology Detection & Control Exchange
On 20–22 July 2017 the 7th Food Microbiology Detection and Control Technology Exchange was held in Shanghai, co-organised by the Agro-product Safety Research Center of the Chinese Academy of Inspection and Quarantine, the Analytical Microbiology Committee of the Chinese Society for Microbiology, and Foodmate.
Read more →
Forum | 7th Food Microbiology Detection & Control Technology Exchange, 19–22 July
Foodborne disease caused by pathogenic microorganisms is the number-one food-safety issue. Controlling contamination, strengthening laboratory capability and applying advanced microbial detection and control technology have become focal concerns for regulators and food companies alike.
Read more →
ZC Bioscience at the 10th China International Food Safety & Quality Conference (CBIFS 2017)
The annual CBIFS food-safety technology forum was held on 7–8 April 2017 at the Hangzhou International Expo Center. ZC Bioscience, a newcomer to food-safety testing, made its debut at the conference.
Read more →
Contact
Talk to ZC Bioscience.
Quotes, datasheets, distributor enquiries and custom development — we reply within two business days.
AddressTianhe Hi-Tech Park, Bldg 5, 688 Bin'an Rd, Binjiang District, Hangzhou 310052, China
Emailzcsw2017@163.com
Phone+86 571 8861 9668Mon–Fri 9:00–18:00 (UTC+8)